Journal article
Cavin4 interacts with bin1 to promote t-tubule formation and stability in developing skeletal muscle
HP Lo, YW Lim, Z Xiong, N Martel, C Ferguson, N Ariotti, J Giacomotto, J Rae, M Floetenmeyer, SV Moradi, Y Gao, VA Tillu, D Xia, H Wang, S Rahnama, SJ Nixon, M Bastiani, RD Day, KA Smith, NJ Palpant Show all
Journal of Cell Biology | ROCKEFELLER UNIV PRESS | Published : 2021
Abstract
The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems, mouse and zebrafish. In both models, Cavin4 localized to T-tubules, and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicated cavin4 paralogs, Cavin4b was shown to directly interact with the T-tubule–associated BAR domain protein Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+ response upon mechani..
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Awarded by Australian Cancer Research Foundation
Funding Acknowledgements
This work was supported by the National Health and Medical Research Council of Australia (APP1156489 to R.G. Parton; APP1099251 to R.G. Parton and T.E. Hall) , National Health and Medical Research Council Emerging Leader Fellowship (1174145 to J Giacomotto) , the Australian Research Council (DP200102559 to T.E. Hall and R.G. Parton) , Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (CE140100036 to R.G. Parton) , and Australian Research Council Centre of Excellence in Synthetic Biology (CE200100029 to K. Alexandrov) . The work was supported in part by CSIRO- Queensland University of Technology Synthetic Biology Alli-ance. We are also grateful to The University of Queensland Major Equipment and Infrastructure Scheme. Confocal micros-copy was performed at the Australian Cancer Research Foun-dation/Institute for Molecular Bioscience Dynamic Imaging Facility for Cancer Biology, established with funding from the Australian Cancer Research Foundation. The authors acknowl-edge the use of the Australian Microscopy & Microanalysis Re-search Facility at the Center for Microscopy and Microanalysis, The University of Queensland. Genome editing was performed by the Queensland Facility for Advanced Genome Editing, Ge-nome Innovation Hub and the Transgenic Animal Service of Queensland, The University of Queensland.